Knowlesi by real-time PCR, 2011-2012

Molecular Detection of Plasmodium knowlesi in a Dutch Traveler by Real-Time PCR:

'via Blog this'

2004 Thailand Knowlesi single case report

Naturally Acquired Plasmodium knowlesi Malaria in Human, Thailand - Vol. 10 No. 12 - December 2004 - Emerging Infectious Disease journal - CDC:

'via Blog this'

sawmill supervisor at Kluang, Johor single case report P.Malaysia

http://www.malariajournal.com/content/pdf/1475-2875-12-88.pdf

'via Blog this'

Plasmodium knowlesi isolate AMIPk001 small subunit ribosomal RNA gene, partial sequence.

GU049678

/organism="Plasmodium knowlesi"
                     /mol_type="genomic DNA"
                     /isolate="AMIPk001"
                     /db_xref="taxon:5850"
                     /PCR_primers="fwd_name: Pmk8, fwd_seq:
                     gttagcgagagccacaaaaaagcgaat, rev_name: Pmk9, rev_seq:
                     actcaaagtaacaaaatcttccgta"

'via Blog this'

pK knowlesi First report from Kalimantan, traveller 2wks.

Plasmodium knowlesi in Human, Indonesian Borneo - Vol. 16 No. 4 - April 2010 - Emerging Infectious Disease journal - CDC:

'via Blog this'

A TaqMan real-time PCR assay for the detection and quantitation of Plasmodiumknowlesi, Malaria Journal 2010,9:344 doi:10.1186/1475-2875-9-344Paul CS Divis (pauldivis@gmail.com)Sandra E Shokoples (sandy.shokoples@albertahealthservices.ca)Balbir Singh (bskhaira55@gmail.com)Stephanie K Yanow (stephanie.yanow@albertahealthservices.ca)

http://www.malariajournal.com/content/9/1/344

sensitive real-time PCR is found to detect pK.

CDC EID Volume 14, Number 5—May 2008
Human Infections with Plasmodium knowlesi, the Philippines

Jennifer Luchavez* , Fe Espino*, Peter Curameng†, Ronald Espina†, David Bell‡, Peter Chiodini§, Debbie Nolder§, Colin Sutherland§, Kim-Sung Lee¶, and Balbir Singh
¶

http://wwwnc.cdc.gov/eid/article/14/5/07-1407_article.htm

At Palawan, by nested-PCR


Why no one use LAMP method? Because it is made in Japan, pricy! 
But the process is much easier and sensitivity is 10x higher..

EBSCOhost: Plasmodium knowlesi malaria in Vietnam: some clarifications.

EBSCOhost: Plasmodium knowlesi malaria in Vietnam: some clarifications.

objection to the denial comment of miss detection by Cox-Singh.